第19回細胞生理学セミナー
本セミナーは、大学院創薬科学研究科・先端薬科学特論 単位認定講義です。
単位認定の出席は各セミナーごとに1カウントで、両⽅出席の場合は2カウントされます。
日時 | 2018年11月07日水曜日・13:00-16:00 |
---|---|
場所 | 創薬科学研究館 2階 205室 |
連絡先 | 大嶋篤典(atsu at cespi.nagoya-u.ac.jp(atを@に代えて下さい。)/6837) |
ファイル | 1539668260Flyer_19th..pdf |
(13:00-14:30)
講演者:
Dr. Dror S. Chorev
Postdoctoral Researcher,
Physical and theoretical chemistry laboratory,
University of Oxford, UK
講演タイトル:
Detergent‐ and chemical‐free native mass spectrometry reveals the protein complex ensemble of whole membrane fractions
講演要旨:
Membrane proteins reside in hydrophobic lipid bilayers and are typically extracted from this environment for study, which often compromises their integrity. This is done by a variety of methods including detergents, amphipols, nanodiscs and more recently by SMALPS. However, these methods remove the protein from its native membrane, therefore altering its environment. Here I present a novel approach that requires no recourse to chemicals, in which protein complexes packed inside membrane vesicles derived from various organisms are transmitted into a mass spectrometer while still inside their native lipid environment. Using this method, new complexes and novel lipid binding properties of a multitude of membrane proteins are revealed.
(14:30-16:00)
講演者:
Christoph Gerle
大阪大学 蛋白質研究所
特任准教授・理学博士
講演タイトル:
Mammalian FoF1 ATP synthase as the mitochondrial permeability transition pore — new drug targets in old proteins
講演要旨:
Since its discovery as the central energy converting enzyme by Efraim Racker, Yasuo Kagawa and colleagues almost 60 years ago, the mitochondrial FoF1 ATP synthase has been studied extensively all over the world and especially in Japan. This large transmembrane super-complex with more than 60 subunits yielded many big surprises, like being powered by electricity, having a rotary catalytic mechanism, the ability to bend membranes and the first horizontal arrangement of transmembrane alpha helices. Now it has been proposed that the FoF1 ATP synthase not only produces the majority of cellular ATP and determines mitochondrial cristae architecture, but that it also is the molecular identity of the elusive mitochondrial permeability transition pore; i.e. the first step of the mitochondrial apoptotic pathway. If true, the FoF1 ATP synthase would suddenly transmutate from a stable housekeeping complex into a major drug target for cancer therapy and age related diseases.
Here I want introduce some of our recent findings on this topic and discuss their possible consequences.
講演者:
Dr. Dror S. Chorev
Postdoctoral Researcher,
Physical and theoretical chemistry laboratory,
University of Oxford, UK
講演タイトル:
Detergent‐ and chemical‐free native mass spectrometry reveals the protein complex ensemble of whole membrane fractions
講演要旨:
Membrane proteins reside in hydrophobic lipid bilayers and are typically extracted from this environment for study, which often compromises their integrity. This is done by a variety of methods including detergents, amphipols, nanodiscs and more recently by SMALPS. However, these methods remove the protein from its native membrane, therefore altering its environment. Here I present a novel approach that requires no recourse to chemicals, in which protein complexes packed inside membrane vesicles derived from various organisms are transmitted into a mass spectrometer while still inside their native lipid environment. Using this method, new complexes and novel lipid binding properties of a multitude of membrane proteins are revealed.
(14:30-16:00)
講演者:
Christoph Gerle
大阪大学 蛋白質研究所
特任准教授・理学博士
講演タイトル:
Mammalian FoF1 ATP synthase as the mitochondrial permeability transition pore — new drug targets in old proteins
講演要旨:
Since its discovery as the central energy converting enzyme by Efraim Racker, Yasuo Kagawa and colleagues almost 60 years ago, the mitochondrial FoF1 ATP synthase has been studied extensively all over the world and especially in Japan. This large transmembrane super-complex with more than 60 subunits yielded many big surprises, like being powered by electricity, having a rotary catalytic mechanism, the ability to bend membranes and the first horizontal arrangement of transmembrane alpha helices. Now it has been proposed that the FoF1 ATP synthase not only produces the majority of cellular ATP and determines mitochondrial cristae architecture, but that it also is the molecular identity of the elusive mitochondrial permeability transition pore; i.e. the first step of the mitochondrial apoptotic pathway. If true, the FoF1 ATP synthase would suddenly transmutate from a stable housekeeping complex into a major drug target for cancer therapy and age related diseases.
Here I want introduce some of our recent findings on this topic and discuss their possible consequences.