Cellular Biochemistry Laboratory, Graduate school of Pharmaceutical Sciences, Nagoya University

原著論文 (Publications)

Takeuchi T^*, Tatsukawa H^*, Shinoda Y, Kuwata K, Nishiga M, Takahashi H, Hase N, Hitomi K.
Spatially resolved identification of transglutaminase substrates by proteomics in pulmonary fibrosis.
Am J Respir Cell Mol Biol. 2021　　In press

Watanabe Y, Oguri R, Suzuki R, Ishikawa Y, Tatsukawa H, Hashimoto H, Hitomi K.
Thrombin-deficient mutant of medaka, a model fish, displays serious retardation in blood coagulation.
Biosci Biotechnol Biochem. 2021 Mar 24;85(4):824-833. doi: 10.1093/bbb/zbaa098. PMID: 32251518


辰川英樹、篠田祥希、竹内大修、人見清隆
タンパク質架橋酵素を介した上皮細胞の間葉転換機構の解析
日本応用酵素協会誌　No. 55　1-9（2020）

Watanabe Y, Okuya K, Takada Y, Kinoshita M, Yokoi S, Chisada S, Kamei Y, Tatsukawa H, Yamamoto N, Abe H, Hashimoto H, Hitomi K.
Gene disruption of medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) causes movement retardation.
J Biochem. 2020 Sep 1;168(3):213-222. doi: 10.1093/jb/mvaa038. PMID: 32251518

Tatsukawa H, Takeuchi T, Shinoda Y, Hitomi K.
Identification and characterization of substrates crosslinked by transglutaminases in liver and kidney fibrosis.
Anal Biochem. 2020 Sep 1;604:113629. doi: 10.1016/j.ab.2020.113629. Online ahead of print. PMID: 32061735

Meng Q, Watanabe Y, Suzuki R, Oguri R, Tatsukawa H, Hitomi K.
Transglutaminase orthologues for medaka fish research model- biochemical characterization and establishment of gene-deficient mutants.
Anal Biochem. 2020 Sep 1;604:113610. doi: 10.1016/j.ab.2020.113610. Online ahead of print. PMID: 32014415

Teshima H, Kato M, Tatsukawa H, Hitomi K.
Analysis of the expression of transglutaminases in the reconstructed human epidermis using three-dimensional cell culture.
Anal Biochem. 2020 Aug 15;603:113606. doi: 10.1016/j.ab.2020.113606. Online ahead of print. PMID: 32004543

手島裕文, 加藤まなみ, 辰川英樹, 人見清隆
皮膚表皮形成を司るタンパク質架橋化酵素・トランスグルタミナーゼ　
シーエムシー出版　食品・バイオにおける最新の酵素利用 井上國世 監修　分担執筆　第14章　(2019)

Tanabe Y, Yamane M, Kato M, Teshima H, Kuribayashi M, Tatsukawa H, Takama H, Akiyama M, Hitomi K.
Studies on differentiation-dependent expression and activity of distinct transglutaminases by specific substrate peptides using three-dimensional reconstituted epidermis.
FEBS J. 2019 Jul;286(13):2536-2548. doi: 10.1111/febs.14832. PMID: 30941897

Ito Y, Tatsukawa H, Yamaguchi H, Takahashi K, Hitomi K, Yuzawa Y.
Detection and identification of potential transglutaminase 2 substrates in the mouse renal glomeruli.
Arch Biochem Biophys. 2018 Dec 15;660:11-19. doi: 10.1016/j.abb.2018.10.001. PMID: 30300608

Mi?ikova I, Pfeffer T, Nardiello C, Surate Solaligue DE, Steenbock H, Tatsukawa H, Silva DM, Vadasz I, Herold S, Pease RJ, Iismaa SE, Hitomi K, Seeger W, Brinckmann J, Morty RE.
Targeting transglutaminase 2 partially restores extracellular matrix structure but not alveolar architecture in experimental bronchopulmonary dysplasia.
FEBS J. 2018 Jun 23. doi: 10.1111/febs.14596. PMID: 29935061

Tatsukawa H, Otsu R, Tani Y, Wakita R, Hitomi K.
Isozyme-specific comprehensive characterization of transglutaminase-crosslinked substrates in kidney fibrosis.
Sci Rep. 2018 May 9;8(1):7306 (18 pages). doi: 10.1038/s41598-018-25674-4. PMID: 29743665

Watanabe Y, Furukawa E, Tatsukawa H, Hashimoto H, Kamei Y, Taniguchi Y, Hitomi K.
Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases.
Biosci Biotechnol Biochem. (2018) Mar 23: 1-4. doi: 10.1080/09168451.2018.1453294. in press PMID: 29570037

辰川英樹、人見清隆
組織の線維化に伴い架橋される基質タンパク質群の網羅的同定・解析　
日本応用酵素協会誌　No. 52　1-10（2017）

Qin XY, Hara M, Arner E, Kawaguchi Y, Inoue I, Tatsukawa H, Furutani Y, Nagatsuma K, Matsuura T, Wei F, Kikuchi J, Sone H, Daub C, Kawaji H, Lassmann T, Itoh M, Suzuki H, Carninci P, Hayashizaki Y; FANTOM consortium, Kokudo N, Forrest ARR, Kojima S.
Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes.
EBioMedicine. (2017) 24: 257-266. doi: 10.1016/j.ebiom.2017.09.008. PMID: 28927749

Noguchi S, Arakawa T, Fukuda S, Furuno M, Hasegawa A, Hori F, Ishikawa-Kato S, Kaida K, Kaiho A, Kanamori-Katayama M, Kawashima T, Kojima M, Kubosaki A, Manabe RI, Murata M, Nagao-Sato S, Nakazato K, Ninomiya N, Nishiyori-Sueki H, Noma S, Saijyo E, Saka A, Sakai M, Simon C, Suzuki N, Tagami M, Watanabe S, Yoshida S, Arner P, Axton RA, Babina M, Baillie JK, Barnett TC, Beckhouse AG, Blumenthal A, Bodega B, Bonetti A, Briggs J, Brombacher F, Carlisle AJ, Clevers HC, Davis CA, Detmar M, Dohi T, Edge ASB, Edinger M, Ehrlund A, Ekwall K, Endoh M, Enomoto H, Eslami A, Fagiolini M, Fairbairn L, Farach-Carson MC, Faulkner GJ, Ferrai C, Fisher ME, Forrester LM, Fujita R, Furusawa JI, Geijtenbeek TB, Gingeras T, Goldowitz D, Guhl S, Guler R, Gustincich S, Ha TJ, Hamaguchi M, Hara M, Hasegawa Y, Herlyn M, Heutink P, Hitchens KJ, Hume DA, Ikawa T, Ishizu Y, Kai C, Kawamoto H, Kawamura YI, Kempfle JS, Kenna TJ, Kere J, Khachigian LM, Kitamura T, Klein S, Klinken SP, Knox AJ, Kojima S, Koseki H, Koyasu S, Lee W, Lennartsson A, Mackay-Sim A, Mejhert N, Mizuno Y, Morikawa H, Morimoto M, Moro K, Morris KJ, Motohashi H, Mummery CL, Nakachi Y, Nakahara F, Nakamura T, Nakamura Y, Nozaki T, Ogishima S, Ohkura N, Ohno H, Ohshima M, Okada-Hatakeyama M, Okazaki Y, Orlando V, Ovchinnikov DA, Passier R, Patrikakis M, Pombo A, Pradhan-Bhatt S, Qin XY, Rehli M, Rizzu P, Roy S, Sajantila A, Sakaguchi S, Sato H, Satoh H, Savvi S, Saxena A, Schmidl C, Schneider C, Schulze-Tanzil GG, Schwegmann A, Sheng G, Shin JW, Sugiyama D, Sugiyama T, Summers KM, Takahashi N, Takai J, Tanaka H, Tatsukawa H, Tomoiu A, Toyoda H, van de Wetering M, van den Berg LM, Verardo R, Vijayan D, Wells CA, Winteringham LN, Wolvetang E, Yamaguchi Y, Yamamoto M, Yanagi-Mizuochi C, Yoneda M, Yonekura Y, Zhang PG, Zucchelli S, Abugessaisa I, Arner E, Harshbarger J, Kondo A, Lassmann T, Lizio M, Sahin S, Sengstag T, Severin J, Shimoji H, Suzuki M, Suzuki H, Kawai J, Kondo N, Itoh M, Daub CO, Kasukawa T, Kawaji H, Carninci P, Forrest ARR, Hayashizaki Y.
FANTOM5 CAGE profiles of human and mouse samples.
Sci Data. 2017 Aug 29;4:170112. doi: 10.1038/sdata.2017.112. in press PMID: 28850106

Horimizu R, Ogawa R, Watanabe Y, Tatsukawa H, Kinoshita M, Hashimoto H, Hitomi K.
Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant.
FEBS J. 2017 Jun 27. doi: 10.1111/febs.14153. in press PMID: 28653811

Tatsukawa H, Tani Y, Otsu R, Nakagawa H, Hitomi K.
Global identification and analysis of isozyme-specific possible substrates crosslinked by transglutaminases using substrate peptides in mouse liver fibrosis
Sci Rep. 7, 45049 (2017) doi: 10.1038/srep45049 PMID: 28327670

Takada Y, Watanabe Y, Okuya K, Tatsukawa H, Hashimoto H, Hitomi K.
Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2).
Biosci Biotechnol Biochem. 81(3), 469-474 (2017). doi: 10.1080/09168451.2016.1256757.

Tatsukawa H, Liu HH, Oba S, Kamiya N, Nakanishi Y, Hitomi K.
FRET-based detection of isozyme-specific activities of transglutaminases.
Amino Acids. 49(3), 615-623 (2017). doi: 10.1007/s00726-016-2322-0.

Yamane M, Sugimura K, Kawasaki H, Tatsukawa H, Hitomi K.
Analysis on transglutaminase 1 and its substrates using specific substrate peptide in cultured keratinocytes.
Biochem Biophys Res Commun. 478(1), 343-348 (2016). doi: 10.1016/j.bbrc.2016.07.051.

Tatsukawa H, Furutani Y, Hitomi K, Kojima S.
Transglutaminase 2 has opposing roles in the regulation of cellular functions as well as cell growth and death.
Cell Death Dis. 7(6), e2244 (2016). doi: 10.1038/cddis.2016.150.

Qin XY, Tatsukawa H, Hitomi K, Shirakami Y, Ishibashi N, Shimizu M, Moriwaki H, Kojima S.
Metabolome Analyses Uncovered a Novel Inhibitory Effect of Acyclic Retinoid on Aberrant Lipogenesis in a Mouse Diethylnitrosamine-Induced Hepatic Tumorigenesis Model.
Cancer Prev Res (Phila). 9(3), 205-14 (2016). doi: 10.1158/1940-6207.CAPR-15-0326.

Kikuta A, Furukawa E, Ogawa R, Suganuma N, Saitoh M, Nishimaki T, Katsumura T, Oota H, Kawamoto T, Tatsukawa H, Hashimoto H, Kiyotaka Hitomi.
Biochemical characterization of medaka (Oryzias latipes) transglutaminases, OlTGK1 and OlTGK2, as orthologues of human keratinocyte-type transglutaminase.
PLOS One. (2015) doi: 10.1371/journal.pone.0144194.

Shrestha R, Tatsukawa H, Shrestha R, Ishibashi N, Matsuura T, Kagechika H, Kose S, Hitomi K, Imamoto N, Kojima S.
Molecular mechanism by which acyclic retinoid induces nuclear localization of transglutaminase 2 in human hepatocellular carcinoma cells.
Cell Death Dis. 6, e2002 (2015). doi: 10.1038/cddis.2015.339.

Tatsukawa H, Abe N, Ohashi S, Hitomi K.
Distribution of transglutaminase family members in mouse whole body sections.
Biochem Biophys Res Commun. 467(4), 1046-51 (2015). doi: 10.1016/j.bbrc.2015.10.001.

Furukawa K, Yamane M, Tatsukawa H, Hitomi K.
Early response as shown by enhancement of transglutaminase 1 expression after cisplatin-induced acute kidney injury.
Arch Biochem Biophys. 586, 27-32 (2015). doi: 10.1016/j.abb.2015.09.021.

Yoshihara M, Ohmiya H, Hara S, Kawasaki S; FANTOM consortium (Tatsukawa H), Hayashizaki Y, Itoh M, Kawaji H, Tsujikawa M, Nishida K.
Discovery of molecular markers to discriminate corneal endothelial cells in the human body.
PLoS One. 10(3), e0117581 (2015). doi: 10.1371/journal.pone.0117581.

Liang C; FANTOM Consortium (Tatsukawa H), Forrest AR, Wagner GP.
The statistical geometry of transcriptome divergence in cell-type evolution and cancer.
Nat Commun. 6, 6066 (2015). doi: 10.1038/ncomms7066.

Hasegawa Y, Tang D, Takahashi N, …, FANTOM consortium (Tatsukawa H), et al.
CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes.
Sci Rep. 4, 5228 (10 pages) (2014)

Morikawa H, Ohkura N, Vandenbon A, …, FANTOM consortium (Tatsukawa H), et al.
Differential roles of epigenetic changes and Foxp3 expression in regulatory T cell-specific transcriptional regulation.
Proc Natl Acad Sci U S A. 111, 5289-5294 (2014)

Forrest AR, Kawaji H, Rehli M, …, FANTOM consortium (Tatsukawa H), et al.
A promoter-level mammalian expression atlas.
Nature. 507, 462-470 (2014)

Andersson R, Gebhard C, Miguel-Escalada I, …, FANTOM consortium (Tatsukawa H), et al.
An atlas of active enhancers across human cell types and tissues.
Nature. 507, 455-461 (2014)

Miura A, Kambe Y, Inoue K, Tatsukawa H, Kurihara T, Griffin M, Kojima S and Miyata A.
Pituitary adenylate cyclase-activating polypeptide type 1 receptor (PAC1) gene is suppressed by transglutaminase 2 activation.
J Biol Chem. 288, 32720-32730 (2013)

Itoh M, Tatsukawa H, Eun-Seo L, Yamanishi K, Kojima S and Hitomi K.
Variations in both TG1 and TG2 isozyme-specific in situ activities and protein expressions during mouse embryonic development.
J Histochem Cytochem. 61(11), 793-801 (2013)

Kuramoto K, Yamasaki R, Shimizu Y, Tatsukawa H and Hitomi K.
Phage-displayed peptide library screening for preferred human substrate peptide sequences for transglutaminase 7.
Arch Biochem Biophys. 537, 138-143 (2013)

Kojima S, Kuo TF and Tatsukawa H.
Regulation of transglutaminase-mediated hepatic cell death in alcoholic steatohepatitis and non-alcoholic steatohepatitis.
J Gastroenterol Hepatol. 27, 52-57 (2012)

Kuo TF, Tatsukawa H, Matsuura T, Nagatsuma K, Hirose S and Kojima S.
Free fatty acids induce transglutaminase 2-dependent apoptosis in hepatocytes via ER stress-stimulated PERK pathways.
J Cell Physiol. 227, 1130-1137 (2012)

Tatsukawa H, Sano T, Fukaya Y, Ishibashi N, Watanabe M, Okuno M, Moriwaki H and Kojima S.
Dual induction of caspase 3- and transglutaminase-dependent apoptosis by acyclic retinoid in hepatocellular carcinoma cells.
Mol Cancer. 10, 4 (11 pages) (2011)

Kuo TF, Tatsukawa H and Kojima S.
New insights into the functions and localization of nuclear transglutaminase 2.
FEBS J. 278, 4756-4767 (2011)

Itoh M, Kawamoto T, Tatsukawa H, Kojima S, Yamanishi K and Hitomi K.
In situ detection of active transglutaminases for keratinocyte-type (TGase 1) and tissue-type (TGase 2) using fluorescence-labeled highly reactive substrate peptides.
J Histochem Cytochem. 59, 180-187 (2011)

Saiki R, Park H, Ishii I, Yoshida M, Nishimura K, Toida T, Tatsukawa H, Kojima S, Ikeguchi Y, Pegg AE, Kashiwagi K and Igarashi K.
Brain infarction correlates more closely with acrolein than with reactive oxygen species.
Biochem Biophys Res Commun. 404, 1044-1049 (2011)

Kojima S, Kuo TF, Tatsukawa H and Hirose S.
Induction of Cross-Linking and Silencing of Sp1 by Transglutaminase during Liver Injury in ASH and NASH via Different ER Stress Pathways.
Dig Dis. 28, 715-721 (2010)

Tatsukawa H and Kojima S.
Recent Advances in the Understanding of Roles of Transglutaminase 2 in Alcoholic Steatohepatitis.
Cell Biol Int. 34, 325-334 (2009)

Tatsukawa H, Fukaya Y, Frampton G, Martinez-Fuentes A, Suzuki K, Kuo TF, Nagatsuma K, Shimokado K, Okuno M, Wu J, Iismaa S, Matsuura T, Tsukamoto H, Zern MA, Graham RM, and Kojima S.
Role of Transglutaminase 2 in Liver Injury via Crosslinking and Silencing of Transcription Factor, Sp1.
Gastroenterology. 136, 1783-1795 (2009)
「表紙に採用（右図）、編集長によるEditorialあり」

辰川英樹、小嶋聡一
アルコール性肝障害の新規肝細胞死誘導経路の発見.
バイオサイエンスとインダストリー. 67(8), 423-427 (2009)

辰川英樹、小嶋聡一
生体内バイオハイブリッド反応−架橋多機能性酵素、トランスグルタミナーゼによるタンパク質の機能変換.
化学工業. 54, 908-915 (2003)

論文紹介記事

辰川英樹
異常な細胞増殖・代謝にかかわるタンパク質の糖鎖修飾.
ファルマシア. 49(7), 701 (2013)

辰川英樹
生きた細胞でのヒストンアセチル化をリアルタイムで観察.
Chemical Biology. 2(3), 20 (2009)

辰川英樹
アテローム性動脈硬化症発症過程における血漿トランスグルタミナーゼ（XIIIA因子）による単球のアンジオテンシン�U受容体（AT1受容体）架橋二量体の形成.
日本血栓止血学会. 16(3), 339 (2005)